目的 建立同时测定香青兰提取物中1种苯丙素类成分(迷迭香酸)和2种黄酮类成分(木犀草素-7-O-β-D-葡萄糖醛酸苷和田蓟苷)的含量测定方法。方法 采用Purospher STARLP RP-18 endcapped(4.6 mm×250 mm,5μm)色谱柱,流动相为乙睛(A)-0.5%甲酸水溶液(B),梯度洗脱(0~28 min,20% A;28~55 min,20%→28% A;55~80 min,28%→30% A),流速为1.0 mL·min-1,检测波长为330 nm,柱温为35 ℃。结果 迷迭香酸、木犀草素-7-O-β-D-葡萄糖醛酸苷和田蓟苷分别在2.184~21.84 μg·mL-1(r=0.999 6),3.14~31.84 μg·mL-1(r=0.999 6),7.9~39.5 μg·mL-1(r=0.999 5)内呈良好线性关系。香青兰提取物迷迭香酸、木犀草素-7-O-β-D-葡萄糖醛酸苷和田蓟苷的平均回收率(n=9)分别为99.62%、99.64%和100.12%,且RSD分别为1.27%、1.05%和1.09%。结论 本方法准确、简便,重复性好。可用于同时测定香青兰提取物中木犀草素-7-O-β-D-葡萄糖醛酸苷、迷迭香酸和田蓟苷的含量。
Abstract
OBJECTIVE To establish an HPLC method for simultaneous detemination the contents of rosmarinic acid (phenylpropanoids),luteolin-7-O-β-D-glucuronide and tilianin (flavonoids) in Dracocephalum moldavica L. Extract. METHODS The samples were separated on Purospher STARLP RP-18 endcapped column(4.6 mm×250 mm,5 μm)by gradient elution with acetonitrile (A)-0.5% formic acid solution(B)(0-28 min,20% A;28-55 min,20%→28% A;55-80 min,28%→30% A)as the mobile phase at a flow ratio of 1.0 mL·min-1. The detection wavelength was set at 330 nm and the column temperature was maintained at 35 ℃. RESULTS The calibration curves of rosmarinic acid,luteolin-7-O-β-D-glucuronide, tilianin were in good linearity over the ranges of 2.184-21.84 μg·mL-1 (r=0.999 6), 3.14-31.84 μg·mL-1(r=0.999 6), 7.9-39.5 μg·mL-1 (r=0.999 5) respectively. The average recoveries were 99.62%, 99.64% and 100.12%with RSD values of 1.27%, 1.05% and 1.09%. CONCLUSIONThe method is reliable,simple, and accurate, and can be used for the comprehensive quality control of Dracocephalum moldavica L. extract.
关键词
香青兰 /
迷迭香酸 /
木犀草素-7-O-β-D-葡萄糖醛酸苷 /
田蓟苷 /
高效液相色谱法
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Key words
Dracocephalum moldavica L /
rosmarinic acid /
luteolin-7-O-β-D-glucuronide /
tilianin /
HPLC
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中图分类号:
R917
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参考文献
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脚注
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基金
国家科技重大专项“重大新药创制”课题(2012ZX09102201-009)
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