香青兰提取物中苯丙素类和黄酮类3个化合物的含量测定

于宁,何承辉,邢建国,帕依曼·亥米提, 曾诚

中国药学杂志 ›› 2015, Vol. 50 ›› Issue (13) : 1138-1141.

PDF(683 KB)
PDF(683 KB)
中国药学杂志 ›› 2015, Vol. 50 ›› Issue (13) : 1138-1141. DOI: 10.11669/cpj.2015.13.014
·论 著·

香青兰提取物中苯丙素类和黄酮类3个化合物的含量测定

  • 于宁1,何承辉1,邢建国1*,帕依曼·亥米提1, 曾诚2
作者信息 +

Simultaneous Determination the Contents of Phenylpropanoids and Flavonoids in Dracocephalum moldavica L. Extract

  • YU Ning1, HE Cheng-hui1, XING Jian-guo1*,Payiman·Haimiti1, ZENG Cheng2
Author information +
文章历史 +

摘要

目的 建立同时测定香青兰提取物中1种苯丙素类成分(迷迭香酸)和2种黄酮类成分(木犀草素-7-O-β-D-葡萄糖醛酸苷和田蓟苷)的含量测定方法。方法 采用Purospher STARLP RP-18 endcapped(4.6 mm×250 mm,5μm)色谱柱,流动相为乙睛(A)-0.5%甲酸水溶液(B),梯度洗脱(0~28 min,20% A;28~55 min,20%→28% A;55~80 min,28%→30% A),流速为1.0 mL·min-1,检测波长为330 nm,柱温为35 ℃。结果 迷迭香酸、木犀草素-7-O-β-D-葡萄糖醛酸苷和田蓟苷分别在2.184~21.84 μg·mL-1(r=0.999 6),3.14~31.84 μg·mL-1(r=0.999 6),7.9~39.5 μg·mL-1(r=0.999 5)内呈良好线性关系。香青兰提取物迷迭香酸、木犀草素-7-O-β-D-葡萄糖醛酸苷和田蓟苷的平均回收率(n=9)分别为99.62%、99.64%和100.12%,且RSD分别为1.27%、1.05%和1.09%。结论 本方法准确、简便,重复性好。可用于同时测定香青兰提取物中木犀草素-7-O-β-D-葡萄糖醛酸苷、迷迭香酸和田蓟苷的含量。

Abstract

OBJECTIVE To establish an HPLC method for simultaneous detemination the contents of rosmarinic acid (phenylpropanoids),luteolin-7-O-β-D-glucuronide and tilianin (flavonoids) in Dracocephalum moldavica L. Extract. METHODS The samples were separated on Purospher STARLP RP-18 endcapped column(4.6 mm×250 mm,5 μm)by gradient elution with acetonitrile (A)-0.5% formic acid solution(B)(0-28 min,20% A;28-55 min,20%→28% A;55-80 min,28%→30% A)as the mobile phase at a flow ratio of 1.0 mL·min-1. The detection wavelength was set at 330 nm and the column temperature was maintained at 35 ℃. RESULTS The calibration curves of rosmarinic acid,luteolin-7-O-β-D-glucuronide, tilianin were in good linearity over the ranges of 2.184-21.84 μg·mL-1 (r=0.999 6), 3.14-31.84 μg·mL-1(r=0.999 6), 7.9-39.5 μg·mL-1 (r=0.999 5) respectively. The average recoveries were 99.62%, 99.64% and 100.12%with RSD values of 1.27%, 1.05% and 1.09%. CONCLUSIONThe method is reliable,simple, and accurate, and can be used for the comprehensive quality control of Dracocephalum moldavica L. extract.

关键词

香青兰 / 迷迭香酸 / 木犀草素-7-O-β-D-葡萄糖醛酸苷 / 田蓟苷 / 高效液相色谱法

Key words

Dracocephalum moldavica L / rosmarinic acid / luteolin-7-O-β-D-glucuronide / tilianin / HPLC

引用本文

导出引用
于宁,何承辉,邢建国,帕依曼·亥米提, 曾诚. 香青兰提取物中苯丙素类和黄酮类3个化合物的含量测定[J]. 中国药学杂志, 2015, 50(13): 1138-1141 https://doi.org/10.11669/cpj.2015.13.014
YU Ning, HE Cheng-hui, XING Jian-guo,Payiman·Haimiti, ZENG Cheng. Simultaneous Determination the Contents of Phenylpropanoids and Flavonoids in Dracocephalum moldavica L. Extract[J]. Chinese Pharmaceutical Journal, 2015, 50(13): 1138-1141 https://doi.org/10.11669/cpj.2015.13.014
中图分类号: R917   

参考文献

[1] The Fascicle of Uygur Medicine of Drug Standard of Ministry of Public Health of the Peoples Republic of China(中华人民共和国卫生部药品标准维吾尔药分册) .Urumqi:Xinjiang Science and Technology Press,1999:73.
[2] FENG C G,LI Q. Review of chemical constituents of Dracocephalum moldavica L. and their pharmacological activities. Chin Tradit Pat Med (中成药),2003,25(2):154-156.
[3] YANG L N, XING J G, HE C H,et al.Chemical constituents of Dracocephalum moldavica L. and their pharmacological activities. World Clin Drugs (世界临床药物),2013,34(4):226-230.
[4] GUO X H,CAO W J,FAN X M , et al.Mechanism and protective effects of tilianin on myocardial ischemia-reperfusion injury in rats. Chin J Exp Tradit Med Form(中国实验方剂学杂志),2013,19(5):168-171.
[5] ZHANG Y J, XU W Q, SHEN X.New progress in the research of rosmarinic acid separation,purification and pharmacological actions. Chin New Drugs J(中国新药杂志),2013,22(4):434-437.
[6] WU J Z, YU J P, ZHAO D L. Progress in the research on rosmarinic acid.Nat Prod Res Dev (天然产物研究与开发), 20S05,17(3): 283-288.
[7] WANG J,WEI N,ZHAO H Z,et al.Comparison of HPLC and UPLC determination of five active components of Radix et Rhizoma Salviae Miltiorrhizae in Qishenyiqi dropping pills.Chin J Pharm Anal(药物分析杂志),2011,31(9):1678-1682.
[8] ZHAO K X, SU D, ZHAO X M,et al.SHPLC Determination of luteoin-7-O-β-D-glucuronide in herbs of Ixeris sonchifolia. Chin J Pharm Anal(药物分析杂志),2010,30(6):1019-1021.
[9] CHEN Y H, JIANG X Z, LUO H M,et al.Simultaneous determination of four flavonoids in Ixeris sonchifolia(Bge.)Hance by HPLC. China Pharm(中国药师),2011,14(3):308-310.
YUAN Y, XING J G, ZHANG Y J,et al.HPLC Determination of tilianinin Dracocephalum moldavica L. Extract. Chin J Exp Tradit Med Form(中国实验方剂学杂志),2010,16(13):68-69.

基金

国家科技重大专项“重大新药创制”课题(2012ZX09102201-009)
PDF(683 KB)

78

Accesses

0

Citation

Detail

段落导航
相关文章

/